ReproductiveBiologyandEndocrinology()13:10
AntoninBukovsky
Abstract
Invitromaturation(IVM)andinvitrofertilization(IVF)technologiesarefacingwithgrowingdemandsofolderwomentoconceive.Althoughovarianstemcells(OSCs)ofolderwomenarecapableofproducinginvitrofreshoocyte-likecells(OLCs),suchcellscannotrespondtoIVMandIVFduetothelackofgranulosacellsrequiredfortheirmaturation.Follicularrenewalisalsodependentonsupportofcirculatingbloodmononuclearcells.Theyinduceintermediarystagesofmeiosis(metaphaseIchromosomalduplicationandcrossover,anaphase,telophase,andcytokinesis)innewlyemergingovariangermcells,asforthefirsttimedemonstratedhere,induceformationofgranulosacells,andstimulatefolliculargrowthanddevelopment.ApretreatmentofOSCculturewithmononuclearcellscollectedfrombloodofayounghealthyfertilewomanmaycausedifferentiationofbipotentialOSCsintobothdevelopinggermandgranulosacells.Asmallbloodvolumereplacementmayenabletreatmentofovarianinfertilityinvivo.Thetransferredmononuclearcellsmaytemporarilyrejuvenatevirtuallyalltissues,includingimprovementofthefunctionofendocrinetissues.FormationofnewfolliclesandtheirdevelopmentmaybesufficientforIVMandIVF.Thenovelproposedinvitroapproachesmaybeusedasasecondpossibility.Infertilityofhumanmalesaffectsalmostahalfoftheinfertilitycasesworldwide.Smallbloodvolumereplacementfromyounghealthyfertilemenmayalsobeeasyapproachfortheimprovementofspermqualityinolderorotheraffectedmen.Inaddition,bodyrejuvenationbysmallbloodvolumereplacementfromyounghealthyindividualsofthesamesexcouldrepresentadeclineofinvitromethodologyinfavorofinvivotreatmentforhumanfunctionaldiseases.Hereweproposeforthefirsttimethatbloodmononuclearcellsareessentialforrejuvenationofthosetissues,whereimmunesystem沙枣子开花金锤锤，我是银川的小回回沙枣子开花金锤锤，我是银川的小回回
